Tasnim Reza

9 Questions 9 Answers 0 Followers

Questions related from Tasnim Reza

Did anyone infect EO771.lmb with lentivirus?

I am trying to infect EO771.lmb cells with lentivirus to express luciferase and Tdtomato. My cells are not expressing anything. I tried spinning the virus for 90 min with media+polybrene, but it...

09 January 2023 2,317 1 View

Spontaneous metastasis of nsg mice reason for early metastasis?

Hello. I have injected breast cancer cells with matrigel in 4th inguinal mammary fat pad of nsg mice. i was not expecting the cells to metastasize before 2 weeks but when I imaged them on day...

01 February 2021 2,943 2 View

Does any one have a protocol to collect CTC from mice whole blood?

Hello, I am doing a cancer metastasis experiment both primary and experimental metastasis. I would like to know has anyone collected CTC from mice blood and how did you store it? Regards Tasnim

09 March 2020 3,098 2 View

Mda Mb 231 cells getting contaminated ?

Hello, I have a 231 cell line growing but it the media is getting cloudy periodically. I have changed the media, PBS, trypsin but still it is getting contaminated. I thought it might be...

09 September 2019 8,241 4 View

Mda Mb 231 cells getting contaminated ?

Hello, I have a 231 cell line growing but it the media is getting cloudy periodically. I have changed the media, PBS, trypsin but still it is getting contaminated. I thought it might be...

09 September 2019 788 4 View

Why Self Circulation of Plasmid DNA not working?

Hello, I am trying to circularize DNA which is 9000 bp long. I am using the thermofisher T4 ligase and their self circulation protocol but I am not getting any colonies. Even if I get one colony...

31 July 2019 8,809 10 View

How do I close a Plasmid after PCR purification?

Hello, I have a plasmid and I have deleted a section containing an undesired DNA. I am producing a control plasmid with everything but the gene of interest. So I do not know how to close the...

06 June 2019 8,016 3 View

Cannot cut a DNA out of plasmid with restriction enzyme?

I have a plasmid of 11242 bp I want to cut out a chunk of it encoding a protein I don't need around 2000 bp. what would be the best way to do that?

02 February 2019 8,213 9 View

How to do one way ANOVA Invasion assay results?

I performed an invasion assay with mutants so I have control and wild type and several other mutants. I want to do one way ANOVA in SPSS I am not sure what should be my dependent or independent...

11 November 2018 1,297 0 View