Dipak Kumar Das

7 Questions 11 Answers 0 Followers

Questions related from Dipak Kumar Das

How to do inactivated of Saccharomyces cerisisae which has protein inside?

Dear all, How to do inactivated of Saccharomyces cerisisae which has protein inside. It is uracil auxotroph. With Regards, Dipak Das

09 July 2024 3,496 3 View

Which media will produce more biomass in saccharomyces cerevisiae?

Dear all, For higher biomass for saccharomyces cerevisiae which media is suitable in shake flask? 1. YPD media 2. Basal salt media and what is the optimum C/N ratio ( 5:1 /10:1). Thanks &...

19 June 2024 2,248 0 View

In Vortex fermentation how aeration and agitation can improve mixing of liquid?

Hi everyone, How aeration and agitation can influence the mixing of liquid in vortex fermenter? what are the other significant factors that can influence the mixing capacity in vortex...

29 November 2022 8,732 1 View

Does anyone has experience with flavivirus (Dengue, Zika,JEV) infection in diploid cell line?

Dear all, I am doing flavivirus infection in diploid cell line. After infection, no CPE are observe till 7 days. In the same time titer is also low(103-104 ccid50/ml). Is this cell line is not...

01 August 2018 2,806 3 View

Problem in not getting visible plaque after staining for zika virus in BHK-21 cell ?

Dear All, I am seeding 5*105 cells/well of BHK-21 in 6-well plate for zika...

28 August 2017 4,871 3 View

What are the basic criteria for filter sterilization in fermenter?

I am facing problem in filter integrity during sterilization with fermenter. Every time after sterilization, integrity test of filter has failed. I am using PTFE membrane filter which are...

30 January 2015 7,173 3 View

What is the optimum pH for Corynebacterium diphtheriae in a fermentor and which pathway is following during cultivation in a fermentor?

My aim is to produce maximum about of toxin in a fermentor by using chemical defined media. But I am not getting my expected result.

04 January 2015 7,015 3 View