Would anyone please have an example of how a whole liver cell suspension (all cell types in there) looks like in the FSC/SSC, and if the distinct populations (e.g. hepatocytes, endothelial cells and kupffer cells) can be clearly distinguished?
We tried to look at a mouse liver cell suspension for the first time but we do not see any clearly defined populations. We also failed to see clear autofluorescence (green) of the hepatocytes.