How to distinguish between bacterial and host DNA in circulation?

09 September 2013 1 3K Report

I am interested in the composition of cell free DNA found in human blood. Where has it come from. Is it waste product from dying or stressed cells in the body? I know it contains human nuclear and mitochondrial DNA but if we find unmethylated DNA here could this have come from bacteria in the gut? Is there a way to distinguish between bacterial, mitochondrial and human ? Any views welcome

Alexander Sasha Vlassov

there is a new MagMax kit for isolation of circulating/cell free DNA from blood. and then using corresponding TaqMan assays or sequencing you can figure which DNA is there- definitely mostly human, but some microbial will be present as well

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