How to dissolve alpha melanocyte hormone (alpha-MSH)for long storage?

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How to avoid polypyrrole deposit on counter electrode?

Hi all, Currently, I electrodeposited polypyrrole on carbon electrode but I got some problem when polypyrrole also deposited on the surface of the counter electrode. The concentration of pyrrole...

05 February 2021 2,103 3 View

How to Extract DNA from dried rhizomes, wood, galls etc. stored for a long time?

Dear Respectable Scientists, I have been trying to extract DNA from above mentioned sources using different CTAB/SDS Buffer Compositions and purifying agents but I am not able to extract DNA from...

12 January 2021 5,899 8 View

How to carry out Fisher's Exact Test in SPSS for multiple Response Dataset?

I have a dataset including Categorical variables and involves multiple response variable. 20% of the cells in cross tab has less than 5 frequencies and hence I cannot carry out a Chi-square Test....

26 December 2020 4,180 3 View

CLOSED Behavioral changes due to Covid-19?

Hello fellow researchers around the globe :) Happy Holidays!! We are conducting a survey on understanding how device usage during everyday life has developed amid the Covid-19...

21 December 2020 5,363 4 View

Does the Chinese navigation satellite system (Beidou) become global or still regional?

I know that this system was regional before, thus covering specific areas not alla the global

09 December 2020 3,343 3 View

Do the exams for students in electronic education have the same credibility in terms of measuring and evaluating learning as traditional education?

Educators around the world face a new challenge of how to conduct exams to assess what students have learned after the shift towards e-learning following the closure of nearly all universities...

21 September 2020 4,180 15 View

What Features Should an Online Exam System Include?

ith the development of Internet technology, online examination has become more Polar because it helps people save a great deal of energy and time. As an effective and efficient method of teaching...

21 September 2020 2,814 12 View

How can heat transfer coefficient on shell side of Shell and tube heat exchange can be calculated using Ansys Fluent?

I have to calculate heat transfer co-efficient on shell side and plot its variation along the length of heat exchanger

07 September 2020 9,078 6 View

How to get rid of negative Pressure? and my pressure readings are not accurate what can be the cause?

I am simulating shell and tube heat ex-changer i have mass flow inlet and pressure oulet (zero gauge) but my pressure readings are not accurate i have tried velocity inlet but results are same....

07 September 2020 7,298 11 View

AST result in spss ?

hello dear microbiologist Am kinda new and still learning SPSS I want to know what kind of tests should I run for AST results I see lots of paper use chi square. And can anyone provide me with...

10 August 2020 9,911 4 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

28 February 2021 274 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View