Neelaka Molagoda

5 Questions 16 Answers 0 Followers

Questions related from Neelaka Molagoda

Asking help to optimize in-vitro lipoxygenase inhibitory assay protocol?

Dear all, I am trying to establish a protocol to assess the in-vitro lipoxygenase inhibition using soybean lipoxygenase (type V). During the protocol, first, we mixed 10 µL of samples with 45 µL...

22 October 2021 574 3 View

What are the simplest staining assays for myoblast differentiation?

Hello, Currently, I am trying to establish new protocols for myogenesis. I am using C2C12 myoblast. Anyone can recommend me a simple staining assay to confirm the differentiation?

19 April 2021 6,701 6 View

What are the basic differences between mouse BV2 microglia and primary microglia cells?

I would like to know the basic differences between mouse BV2 microglia and primary microglia cells especially, with respect to morphological differences.

18 May 2020 9,512 7 View

What are the special tips for taking clear images of tube formation in angiogenesis?

Recently, I tried to take some images of tube formation in angiogenesis using EA.HY 926 cells. I used Matrigel coated 96 well plate and D-glucose was used as the angiogenic factor. I noticed that...

28 November 2019 4,205 2 View

Can anyone help me with the differentiation of RAW 264.7 cells to osteoclasts?

I can not see any osteoclasts in my culture till day 7 and I am using RANKL from abcam (ab129136).

03 May 2019 7,022 8 View