How to differentiate Peribiliary gland cells from Bile duct cells?

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Are there multiple splice variants of PIEZO-1?

We tried three different piezo-1 Ab's for western blot and we observe four bands 1. 250KDa+, 2. 100Kda, 70 KDa and 30 KDa. PIEZO-1 full length protein should only gibe 250+ KDa band. I would like...

09 October 2018 4,280 4 View

Can someone suggest a good review or book chapter to understand various types of calcium channels ?

I was looking for a generalized view of the various calcium channels such as voltage gated, mechanosensitive calcium channels. Can anyone suggest a good recent review or book chapter which covers...

05 June 2018 7,172 5 View

Red dye for calcium imaging?

Can anyone suggest a good membrane permeable fluorescent dye for imaging intracellelar calcium which has emission either in red or far red region? Most of the good calcium sensitive dyes are green...

04 May 2018 4,578 2 View

Any simple method to correlate two curves?

I am calculating 2 parameters and I see both of them are correlated from graph (The dip in blue curve follows dip in orange curve, see attachment). I was wondering , if there is a mathematical...

04 May 2018 9,919 3 View

Has anyone attended european calcium society meeting?

I was searching for a conference on calcium signalling in cells and came across this meeting https://www.ecs2018.eu/registration.html . The meeting seems good meeting, but with all the predatory...

03 April 2018 5,439 4 View

Which Scopus Journal provides the most affordable fees?

"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....

10 August 2024 8,621 1 View

Seeking Advice on Viability and Execution of Undergraduate Thesis Topic?

Hello everyone, I am currently developing a thesis proposal and would appreciate your input on its viability and how to effectively carry it out. My proposed topic is: "Does the perceived threat...

10 August 2024 8,992 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Who will be moral responsible for the death of thousands of people in the event of an earthquake?

Who will bear moral responsibility for the deaths of thousands of people in the event of an earthquake? Weeks and months remain before the onset of strong earthquakes that bring death to...

08 August 2024 6,134 12 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Are there any instruments for studying time similar to the way it is in space?

There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...

06 August 2024 7,102 0 View

In the case of a wound l recurrence after radical breast cancer and sentinel lymph node biopsy. Are the sentinel lymph node procedure recommended?

In the case of a wound l recurrence after radical breast cancer and sentinel lymph node biopsy. Are the sentinel lymph node procedure recommended? If no axillary lymph node dissection was not...

05 August 2024 8,056 1 View