How to differentiate between confounders and effect modifiers in the articles ? and is it important to be reported in the articles?
Dear Sir I welcome all researchers specialized in the field of artificial neural networks to conduct joint research in this specialty and its applications in engineering and science. If you would...
24 June 2023 8,262 0 View
what is the difference between a covariate and a confounder?
15 June 2023 6,970 3 View
Distinguished researchers and scientists, I hope you all are doing well. In which month of 2023 will the JCR impact factor and quartile ranking of journals be released? thanks
07 June 2023 4,117 2 View
about mutation
20 May 2023 3,238 2 View
Hi everyone, I am in search for positive and negative controls for some of Histone marks for ChIP-qPCR in HUDEP cells. Can anybody help in this matter? Marks are: H3K27ac H3K27me3 H3K4me3...
12 March 2023 5,833 2 View
if a protein has low intracellular expression levels, it is likely that the amount of protein secreted into the extracellular space will also be low?
06 March 2023 6,731 6 View
Good day, If protein expression in blood monocytes was low (detected by western blot) and protein level in serum (detected by ELISA) was high? what dose it mean? Note: the protein should not...
22 February 2023 8,504 6 View
if gene expression is low in specific diseases based on previous research, But when I measured the protein expressed by the same gene in blood of same disease by ELISA I found high serum level?...
21 February 2023 320 4 View
so how to prepare input file in ORCA manually,but ORCA does not have the COSMO solvation Moddel.please help me
03 February 2023 6,198 1 View
Is there any specific reason that we cannot get the transferred colonies? According to our methods and protocols, we have done all the procedures of bacterial transformation and used plasmid pUC19...
11 November 2022 6,102 1 View
I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...
23 July 2024 8,492 3 View
Background: I am developing an RdRp assay for Dengue virus-3 and need to express and isolate the NS5 protein's RdRp domain (~101 kDa). My expression vector includes a GST tag followed by a 6x His...
13 July 2024 9,950 3 View
Have been trying to induce THP-1 using PMA at different concentrations (range from 5 to 50 ng/mL) but after 48h all the conditions except the control are mostly dead, checked by trypan blue and...
03 July 2024 3,884 5 View
I have already cloned ALP gene of L rhamnosus into pET22b+ vector. Cloning is already confirmed by restriction digestion and sequencing. Cloning is achieved in BL21(DE3), Codon+, Rosetta and Lemo...
02 July 2024 5,332 3 View
Has anyone tried co-culturing and SH-SY5Y cells with human astrocytes and differentiating ? I am planning to seed astrocytes, let them grow for 3 days and introduce SH-SY5Y cells into it. Ideally,...
01 July 2024 243 2 View
I have been looking at papers for protein expression and trying to follow the process. I'm using TB medium to do the process, and the paper says to grow to an OD600 = 1.8 at 37 degrees, then let...
30 June 2024 6,502 5 View
Hello, what THP-1 differential markers do you use in the Western-blot method? I am looking for proteins that are characteristic of M0, M1 and M2. Do you have something proven and reliable? Greetings
24 June 2024 9,025 0 View
Dear fellow researchers, We are conducting an ELISA with the "Cattle IGF 1 (Insulin-like growth factor) ELISA Kit" (Cat: ELK5630). Samples and reagents were prepared following the instruction...
13 June 2024 9,473 0 View
Hello esteemed scientists, I am endeavoring to increase the yield of my recombinant protein. It is a 120 kDa protein tagged with MBP and features three intrinsically disordered regions with pI...
12 June 2024 9,616 8 View
We're using LB media, 1mM IPTG, and letting it incubate at 18°C over night. Will adding glucose or something else to the media increase bacterial expression?
06 June 2024 6,071 1 View