Is there any specific reason that we cannot get the transferred colonies?
According to our methods and protocols, we have done all the procedures of bacterial transformation and used plasmid pUC19 on ampicillin plates, but after overnight and even more days of observation, we cannot see any colonies on the ampicillin plates. We repeated several times but still could not get the target points, which are attached here.
Please provide scientific suggestions and solutions; it means a lot to me.
So it is clear that something is not working in your procedure, so you need to do controls and confirmations. The likely things to suspect are:
1) your plates are incorrect (someone added the wrong antibiotic)
2) your plasmid is wrong or there is no plasmid DNA
3) your cells are not competent or are dead
4) you are not following the protocol properly
Most likely the answer is 3, but all should be checked and confirmed until you find the problem.
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