Controls for ChIP-qPCR?

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How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under op microscop?

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under optical microscope?

07 August 2024 2,132 3 View

Unusual intensity drop in some sections of chromatograms in DDA?

Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...

07 August 2024 3,218 4 View

Can you suggest reliable sources defining "3D mesh" and "3D city models"?

Dear fellow researchers, I am currently working on a paper where I need to provide a reliable reference that defines and distinguishes between 3D mesh models and 3D city models. Although I am...

06 August 2024 9,986 2 View

Absorption coefficient of methane?

Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?

06 August 2024 980 5 View

What is the best sampling strategy?

I am conducting a qualitative study that uses interviews to investigate the perceptions of teachers about a particular leadership practice and I am focusing on 3 schools which have a total number...

01 August 2024 8,457 10 View

Looking for help on sem image analysis?

Hello I am conducting a microstructural analysis of a soil treated with lime. The following sem images are of the untreated s1 and treated soil s3. The untreated soil contains quartz calcite...

01 August 2024 572 0 View

What is Random Audit?

HI there, I've came across several articles discuss about random audit an Non random to tax evasion or compliance. Most of the articles is relating about effect of audit (random or non random)...

31 July 2024 5,309 7 View

Can we patent a process flow diagram developed using a process simulator but no actual cases is carried out?

Can we patent a process flow diagram developed using a process simulator but no actual cases is carried out? For example consider a process for certain product manufacture where a new process flow...

31 July 2024 781 1 View

How can we calculate the percentage of configuration interaction (CI) in the UV output data of the Gaussian program?

How can we calculate the percentage of configuration interaction (CI) in the UV output data of the Gaussian program? for example: Excited State 17: Singlet-A 5.1359 eV 241.41 nm...

28 July 2024 9,165 2 View

Please, what is the memory consumption of the Matlab function quad tree decomposition procedure [S = qtdecomp(I)] with respect to the input set I?

27 July 2024 5,455 2 View

How does one get and add a DOI to an existing file upload?

to "Familiar biological, chemical and physical events credibly evolve the Standard Genetic Code", for example?

06 June 2024 5,095 1 View

Why am I having huge shifts in reference genes Cq's?

I was recently performing a great deal of qPCRs on adipose tissue derived RNA. I had experimental samples from sick, and control samples from healthy individuals. I used the same chemistry for all...

04 June 2024 6,924 0 View

How to optimize issues with uneven loading control while doing western blot?

I have quantified the lysate by bradford method and loaded 25ug of lysate for SDS PAGE and proceeded for western blot. However, upon detection I see a variation in loading control i.e., GAPDH. I...

15 May 2024 3,433 6 View

Can I apply two-step custer analysis of continuous variables for defining cases and controls?

There are 33 items adding up to an overall value for qol score. How to present the results of two-step cluster analysis in the methodology for differentiating cases and controls group? Should any...

14 May 2024 7,717 2 View

How to do CUT and TAG normalisation for EZH2 inhibited cells in H3K27me3 pulldown samples?

Dear all, I have performed CUT&TAG and tried to pulldown H3K27me3 containing nucleosomes in the cells treated with EZH2 inhibitor. I spiked my human cells with 20% of Drosophila cells. As...

21 April 2024 3,197 0 View

Lentivirus and HEK293T cells?

i need some help setting up my culture plate having negative controls,I am going to infect HEK293T cells in a 6-well plate... to one well I will add my lentivirus particles(packaged already), and...

15 April 2024 7,218 5 View

Can I use the minus standard deviation to set a positive cutoff?

I am optimizing an indirect ELISA for a flavivirus. All my samples are suspected cases. I have the mean and standard deviations of positive controls. When I use a positive standard deviation,...

14 April 2024 1,277 2 View

Can anyone advise me on epigenomic analysis?

I'm new to epigenomic analysis and have confirmed the high chromatin accessibility of a gene in tissue cells via ATAC-seq. However, I'm unsure about the specific histone modification or DNA...

13 April 2024 7,086 0 View

How can I manage the carriers connected to ePWM for code generation using DSP controller to run the MMC five-level output voltage prototype?

I'm currently facing difficulties in conducting my experiment, which aims to investigate the MMC 5-level output voltage using a DSP controller and MATLAB ePWM signals. I would appreciate your...

09 April 2024 3,371 0 View

Why did someone average the control when performing qpcr calculations?

Why did someone average the control? When selecting your calibrator sample you can select just one of the ‘controls’ that way only 1 sample in the experiment will have a 2^-ddCT of 1.I see a lot...

06 April 2024 3,867 0 View