if gene expression is low in specific diseases based on previous research,
But when I measured the protein expressed by the same gene in blood of same disease by ELISA I found high serum level?
what is the explanation for this difference between gene expression and its protein concentration in blood?
Hello Mirna K. Faiq
Not necessary. In mammalian cells, mRNAs are produced at a much lower rate than proteins are. On an average, a mammalian cell produces two copies of a given mRNA per hour, whereas it produces dozens of copies of the corresponding protein per mRNA per hour. Similarly, mRNAs are less stable than proteins (with an average half-life of 2.6–7 hours versus 46 hours, respectively). Also, changes in gene expression level are frequently not reflected at the protein level.
For more information you may want to refer to the article attached below.
Article Insights into the regulation of protein abundance from prote...
Best.
Malcolm Nobre thank you very much dear doctor, I appreciate your answer.
Dear Mirna K. Faiq
There is no certainty in this matter. There can be other reasons to increase the expression of that protein and even the mRNAs are less stable in comparison with proteins.
Not necessarily. The level of gene expression does not always directly correlate with the amount of protein produced. There are many factors that can affect protein expression, including post-transcriptional modifications, translation efficiency, protein stability, and degradation.
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