How to differenciate between DNA in HBV particles and ldsDNA (integrated) at sequence level?

28 October 2018 1 2K Report

I have fasta files mapping to HBV after sequencing, I'd like to know if there's any difference at sequence level between DNA found in viral particles and DNA integrated in the host?

Patricio Flores-Herrera

Hi Axel!. That depends on how the library was constructed before sequencing, but, the ldsDNA integrated reads should have remaining host dna sequences linked on the 5' and 3' ends. The DNA that comes from the HBV particles should have only the adapters in each ends.

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