To transfect large SV40 T antigen into the patient-derived tumor cells

Surely, tumor cells from the tumor site in patients are lack of normal function of p53, the immortalization is essential. 

Dear Go,

Would you be so kind in sending me a good reference (high impact journal) about your response.

I do not understand why a cancer cell line derived / established from a patient needs to be transfected for p53.

It seems to me that this process can modify the behavior of the cell line.

Dear Yogesh,

Why are "new cell lines" needed according to the fact that hundreds of them are already well characterized (see the attached article).

Best regards

Robert 

thank you sir