As with the standard procedures of determining level of nitrite/nitrate in phytoplankton culture medium before and after experiment, I wish also to determine the level of either vitamin B1 or vitamin B12 in the medium.
A quick search on the internet revealed this process generally required HPLC, which I do not have.
Is that other simple/rapid method known for determining level of vitamin B in liquid?
P/S: I'm referring to the f/2 culture medium of 30 ppt salinity for planktonic dinoflagellates.
Thanks.
Hello,
I am no expert here but I am equally interested for an answer to your question. There is an indirect method using growth of bacteria which require cobalamin for their growth but I am not sure how applicable it is in your case. See attached file.
All the best
Panos
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