Governance issues please?

Please suggest the means for tracing and prioritizing major governance issues in Indian agriculture... Quantification issues as well are welcome... Thanks in advance...

27 December 2020 3,585 3 View

Meta analysis of "proportions" and "odds ratio" - How do you merge data?

Hello everyone.. I am trying to do a meta analysis of studies which report Odds Ratio (OR). I also have some studies which have reported only "proportions" and not OR. My question is - can i...

28 June 2020 3,951 5 View

Using islets immediately upon isolation?

Hello. Our incubator for primary cultures is broken but I need to isolate some islets for both qPCR and estimating the insulin content. Has anyone performed either of these techniques on freshly...

18 November 2019 1,352 3 View

How to get a River basin shape files of India? kindly suggest any?

Please suggest any websites that providing Indian River basin shapefiles.

25 September 2019 7,278 2 View

Is their any mathematical equation to correlate density with kinematic viscosity and calorific value?

can any one suggest mathematical equation for predicting calorific value and kinematic viscosity through density

16 December 2018 3,013 3 View

What is the exact difference between linear and non linear burgers rheological model in application point of view?

As per my search I found that non linear burger's model describes nose criteria of nano indentation better than linear model. please explain other prospects where these are having different...

09 August 2018 8,069 2 View

While Doping what is happening to the XRD pattern of Parent Compound? if it is possible of showing both parent and doped compound same XRD pattern?

In the figure, peaks are matching to the parent compound and the excess peaks are matching for Doping compound. Doping x= 0.2 in parent compound.

13 March 2018 9,130 4 View

Can I get a protocol to isolate supercoiled plasmid DNA?

Acid phenol extraction seems to be feasible in my lab (A/c to the facilities available). I could not find out the protocol to fish only the supercoiled form of plasmid. Please shed some...

22 January 2018 563 3 View

What are the pre-requisites for learning SAM construction & CGE modelling?

04 October 2017 3,593 3 View

NMR reference for fatty acid sugar esters?

Has anyone isolated and characterized simple straight chain fatty acid sugar esters from plants? Kindly share your experiences.

12 May 2017 1,338 1 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How to classify strictly upper triangular matrices by similarity?

Let F be a field. Consider U, the set of n times n strictly upper triangular matrices in F. For X, Y in U, we call them similar if there exists some S, which is non-degenerate and upper...

01 March 2021 2,957 8 View