What do the casual basis of disease and normative basis of disease refer to?

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Does DNase I have lower activity on supercoiled DNA?

Hello everyone, I am working on the production of IVT mRNA. After transcription, the remaining DNA templates are digested by DNase I. I have tested transcription with uncut DNA (mostly...

10 April 2024 1,362 2 View

Can someone recommend primary and secondary antibodies for IHC sclerostin stain?

I have human, non-union tissues to be stained for sclerostin. The tissues were collected in 10% formalin and processed via paraffin embedding. Any recommendations for antibodies are much appreciated

08 April 2024 5,584 0 View

Help dissolving solid drug in aqueous buffer (IP Injection)?

I am working on a pilot study giving rats IP injections daily with a target dosage of 60mg/kg of a drug (BLZ-945). According to product sheet, soluble in organic solvents like ethanol/DMSO/etc....

28 December 2023 4,603 0 View

For gibson assembly, does the linearized vector need blunt ends?

I am looking to amplify a gene from a separate plasmid and insert into a plasmid. the plasmid i will cut with a restriction enzyme that gives sticky ends! Do I need to use T4 polymerase to blunt...

16 April 2023 2,280 3 View

Multiple rounds of electroporation with E coli?

Can E coli cells undergo multiple rounds of electroporation? And does this depend on what strain? The cells are electrocompetent and I will need to do multiple rounds but couldn't find info on...

06 February 2023 3,292 2 View

Does anyone have experience working with T3-free media for mammalian culture--specifically neuron culture?

Hello all, I am currently trying to culture cortical neurons in media free of T3 in hopes to observe change when supplementing the cells with different concentrations of T3. I have looked up some...

04 August 2021 4,426 2 View

To what extent do typically developing children change over time on SDQ scores?

I am conducting research which involves measuring change over time in SDQ scores (parent/teacher/child) in children aged 7-11 years who are identified as at-risk of SEMH difficulties due to...

11 July 2021 4,600 1 View

Is NHS soluble in dichloromethane?

I am trying to dissolve 0.1 g of N-Hydroxysuccinimide in about 5 mL of dichloromethane in a round bottom flask with a magnetic stirrer. I cannot get the NHS to dissolve unless I add 30 mL of...

19 November 2020 9,262 1 View

Does anyone know of a marker expressed on all lymph-resident cells that can be used in flow cytometry?

I am looking for a marker found on all cells that reside in the lymph node - I study the immune response in salivary glands and we want to gate out any lymph resident cells to ensure that there is...

13 October 2020 6,500 2 View

Anyone knows how to not show DOI in Mendeley DESKTOP that they don't show up in the bibliography with app style?

I mean if DOI are in the desktop they always show up in APA even ought I edited the style. I have used the removing DOI process explained on Elsevier website (To do this, using the Style Overview...

09 August 2018 1,524 32 View

• What the possible Persistent Organic Pollutants and Heavy metals present in fluorspar, sediments, and water bodies around its mining area?

Approximate concentrations are require in compared with the WHO permissible limts

11 August 2024 2,723 1 View

Determining the worth of a point improvement in Hamilton Depression Scale?

Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...

05 August 2024 3,246 1 View

Why wait for a doctor's visit when you can become the guardian of your child's health today?

Join our exclusive WhatsApp group and access the latest research-based videos on children's health! Discover the hidden toxins and other risks that may harm your kids, and learn the best practices...

04 August 2024 1,264 0 View

Ready to take control of your child's health and well-being?

Join our exclusive WhatsApp group and gain access to the latest research-based videos on children's health! Uncover the hidden toxins and other risks that may threaten your kids, and equip...

04 August 2024 5,895 0 View

How to report results of Generalised Linear Mixed Models in a journal article?

Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...

04 August 2024 4,130 4 View

How Social Media Affects Your Mental Health ?

How Social Media Affects Your Mental Health

04 August 2024 6,961 3 View

How can I find access to heavy metal reference doses from the EPA and WHO websites?

Reference dose and Maximum acceptable concentrations HMs

03 August 2024 8,230 4 View

Does exposure to toxins during pregnancy increase early puberty risk?

I am currently researching the impact of environmental toxins on children's health and would greatly appreciate insights from experts in the field. If you are an expert or researcher working on...

02 August 2024 8,361 0 View

How do environmental toxins interact with genetic predispositions to increase cancer risk?

I am currently researching the impact of environmental toxins on children's health and would greatly appreciate insights from experts in the field. If you are an expert or researcher working on...

02 August 2024 2,627 1 View

How does social media influence people's dental habits and interest in aesthetic dental treatments?

Hello, Dear friends, I am surveying as part of my thesis research and would greatly appreciate your participation. The study investigates how social media influences dental health behaviors...

01 August 2024 9,349 2 View

Michael Huen Sum Lam

You may want to check this

https://plato.stanford.edu/entries/health-disease/

Martin Hofmeister

Zajicek G. Normative medicine. Med Hypotheses. 1995;45(4):331-4. https://eurekamag.com/pdf.php?pdf=009107328

Hofmann B. Complexity of the concept of disease as shown through rival theoretical frameworks. Theor Med Bioeth. 2001;22(3):211-36. https://www.ansatt.hig.no/bjoernh/Artikler/concept5.pdf