How two melting peaks in DSC of polypropylene can be merged into one crystallization peak?

The figure shows DSC cooling (blue) and 2nd heating (green) curves (step: 10 K/min) for a commercial sample supplied as Atactic Polypropylene (APP). The heating curve illustrates two major...

03 March 2021 5,857 3 View

High aspect ratio hexahedral mesh optimization solver?

I am interested in evaluating mesh optimization solvers that converge to high aspect ratio unstructured hexahedral grids. In other words, I am interested in a solver that optimizes warpage,...

26 February 2021 7,279 2 View

Do you have an example of an algebraic Hamiltonian vector field which possess a finite number of periodic orbits?

Is there a polynomial Hamiltonian vector field with a finite number of periodic orbits? Please see this MO question:...

21 January 2021 1,673 4 View

Does anyone here uses the Software for Roche LightCycler 2.0 instrument?

Hi everyone, If anyone here uses Roche LightCycler 2.0 instrument for qPCR experiments, I would appreciate if they message me. I have some questions about it. Best - Navid

12 October 2020 6,234 2 View

What is an acceptable FDR% for proteomics data publication?

Is there an established convention on this? I have heard from experts that 1% FDR is recommended. I was wondering can we report protein IDs at 5% FDR. Is there any published guidelines on this? If...

25 August 2020 1,231 4 View

Acetic acid conversion to ethanol by LiALh4?

How can I manage acetic acid conversion to ethanol by LiAlH4 safely? I want to do this reaction in laboratory. I have read some text about the reaction condition but I need some more detail to do...

22 August 2020 6,121 1 View

Does the expiration date affect the binding capacity of Ni-NTA?

Hello! I have some expired Ni-NTA resin (QIAGEN), and I need to purify my His-tagged protein. Does the expiration date affect the binding capacity of Ni-NTA? Thank you in advance

11 June 2020 5,773 5 View

How can concrete be modeled with a GFI in ABAQUS?

Hello dears I am modelling concrete in the Abaqus and need to know GFI behavior and parameters them. How can concrete be modeled to the ABAQUS? Thanks

03 March 2020 4,116 3 View

Is there any good free software to simulate electron trajectory in non-uniform electric field?

Need to simulate electron trajectory in non-uniform electric field.

08 February 2020 8,963 3 View

New project in Cathodic Protection?

What fields (topics) do you suggest to work as a metallurgy and Technical Inspection master degree Student in Cathodic Protection? Thank you kindly

17 January 2020 4,201 5 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View