I am using M270 strepatividin magnetic bead to conjugate with biotinylated protein or RNA. How can i detect that my biotinylated protein or RNA is conjugated with streptavidin magnetic beads ?
Dear Enamul Haque,
After you finish with your protocol to conjugate your biotinylated protein on the streptavidin magnetic beads I guess you will store the magnetic beads in a different buffer. To make sure that your biotinylated protein are conjugated to your beads you can try to use the supernatant of your "reaction buffer" and measure the concentration of the undbound biotinylated protein in this buffer (there are different ways to do this, depending on the devices available in your lab). You can compare it to the concentration that you added at the beginning of the conjugation process to see if the protein did bind to the beads. You can also repeat this with your storage buffer if you want to make sure that there is no loss of biotinylated proteins on the surface of the magnetic beads over time.
Best regards,
Benita
In many cases, the quantification of bound reagent does not help you much. It is more relevant to perform a functional test with the beads. If this test fails, it is rarely the streptavidin/biotin interaction, which is the problem.
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