Dear all,

At the moment, I am culturing murine primary keratinocytes on a 3T3 feeder layer (MC treated). By just subculturing the whole culturing by detaching with 0,25% trypsin in 0,03% EDTA, all cells are subcultured and I have fibroblast-contamination (obviously).

In the literature, I read that these fibroblasts can be detached from the culture by treating them with 0,03% EDTA. However, the feeder cells do not detach at all. Is there a trick that I am missing out on?

Looking forward to your experiences!