How to detach 3T3 cells in a 3T3 feeder layer - keratinocyte system?

Dear all, At the moment, I am culturing murine primary keratinocytes on a 3T3 feeder layer (MC treated). By just subculturing the whole culturing by detaching with 0,25% trypsin in 0,03% EDTA,...

27 November 2019 5,383 1 View