I WANT TO DESIGN primers for microRNA
Hi,
If you are trying to quantify a particular miRNA for gene expression profiling using qPCR, then there are commercially available kits help you in cDNA synthesis and RT-qPCR. Life Technologies has variety of those kits based on Taqman chemistry.
Regards
i have designed a primer but i found it gives 200 hits over than the intended sequence (5 of them in the organism that i work with). this give poor pcr results. i want to avoid this but i don,t...
08 September 2018 7,012 3 View
i had designed primers for two sequences using blast tools in NCBI but they gave poor amplification lower than the ladder after gradient pcr i want any one to check them and help me in designing...
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i do a study for a gene expression in HCC patients compared to healthy person using real time pcr. i have analyzed ct for gene of interest and house keeping gene in each sample either HCC or...
06 July 2018 7,002 13 View
I have used the following procedures but am still having strong primer dimer bands while the desired product is absent. The cycling conditions for PCR program that i used were 5 min at 95C for...
02 March 2018 6,091 6 View
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i want to make haplotype and linkage analysis. i found that the most common programme is the haploview. but i don,t know how to make pedigree or linkage or premakeped file to use in the haploview....
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After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
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I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
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Molecular docking software/ websites?
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