Layered structure creation in VESTA?

Hello everyone, I am trying to simulate Ag diffusion through glassy amorphous materials. I have simulated a glass structure using melt-quench technique in VASP (AIMD). After the melt-quench once...

26 November 2019 2,453 1 View

How can I learn HLM ?

09 February 2018 2,250 4 View

Why in HIV /AIDS, CD4:CD8 ratio is considered? Why not CD8:CD4 ratio?

11 January 2017 2,189 3 View

How does a sea of ultracold neutrons interact each other?

I am interested in table top experiments involving probes for strong force. I am an experimentalist and am looking for the answer to the following question- if I have a sea of ultra cold neutrons,...

01 February 2014 320 8 View

Anyone have experience doing HLPC for rebaudioside a from stevia?

What do you thing about this method using Agilent ZORBAX Carbohydrate column? Do you think its better for me to use longer column?

15 November 2012 242 5 View

Has anybody encountered a Tafel like the attached image while measuring corrosion, and do you know the cause of this?

I am running corrosion experiments using a Princeton Applied Research VersaSTAT 4. My system is a carbon steel specimen in a gas treating amine blend loaded with CO2 at 40C. Most trials yield an...

01 January 1970 544 9 View

What should be the pipe materials for measuring vibration from Acoustic Sensors?

Metal or PVC plastic pipe? how can the pipe materials affect measuring the vibration of fluid that is flowing through the pipe?

01 January 1970 775 3 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Genescan gives 3 peaks instead of one?

Hi all, I am doing a genescan analysis for a deleted exon in the patient sample. The amplified region is 215 bp. In the patient sample because of homozygous deletion, no peak is observed while in...

23 February 2021 5,645 1 View

QPCR reaction efficiency with odd results what does this indicate?

I am working on a CFX 96 machine to determine reaction efficiency of my gene of interest. After performing a serial dilution of my cDNA template, I was able to find great reaction efficiencies for...

23 February 2021 809 3 View