Kathan Munshi

2 Questions 3 Answers 0 Followers

Questions related from Kathan Munshi

Is full length cDNA required for making an expression construct or just the coding region would do?

I am cloning a mouse gene under a tissue specific promoter. The vector has polyA tail. In this case is a full length cDNA of the gene necessary of just the CDS would suffice. I am working on PCR...

03 January 2023 2,363 7 View

How to design primers for genes with high AT content?

I am designing primers for ChIP qPCR. One of the genomic region has high AT content. I am not getting any primers for amplicon length of 75-160bp. I even tried getting primers with low Tm. How do...

12 March 2020 7,800 2 View