How to design primers for 5' and 3'-RACE?

14 June 2016 1 2K Report

I want full length CDS of my desired gene. I have partial sequence with me. Thus, I have to perform 5' and 3'-RACE. Can anyone tell me how to design primers for the same?

Emmanuel Delwin Abarike

I think the attached should be of help.

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Science method

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