Varun Kumar

2 Questions 7 Answers 0 Followers

Questions related from Varun Kumar

How to design primers for 5' and 3'-RACE?

I want full length CDS of my desired gene. I have partial sequence with me. Thus, I have to perform 5' and 3'-RACE. Can anyone tell me how to design primers for the same?

14 June 2016 1,672 1 View

How can I determine the copy number of genes from transcriptomic data?

We have generated transcriptomes of various plant tissues. For validation of gene expression, we need to select correct transcript through screening of paralogs, how can we resolve this problem....

21 January 2016 378 11 View