Thanks Rohan, that's exactly what I'm worried about. Thanks for your advice. Our diagnostic lab bought the Ion Torrent, but we may be able to find another way.

Does increasing the depth help with the errors?

I'd love to hear from anyone using the Ion Torrent for panels.

Thanks Rohan. We are looking to sequence whole genes, so not keen on finding too many errors. We have an illumina on site we may be able to use instead.

We have experienced lots of errors with IonTorrent too, both with whole exome and targeted gene panels. One and 2 base indel errors are a real hassle.

Thanks Gianina. Are you still using the IonTorrent for panels or have you moved on to another platform?

I am not a salesperson, but I have used this panel in my research.

http://res.illumina.com/documents/products/datasheets/datasheet_trusight_inherited_disease.pdf

Thanks Casey, we are looking at dominant, adult onset conditions to start with, rather than the recessive, pediatric conditions the trusight is designed for.

From the outset we have been considering whether we can use an Illumina platform, with Ion Torrent as confirmation on another platform (or vice versa). Perhaps to use Rohan's metaphor this is "using a sword for knitting fine silk". Has anyone else attempted this method of confirmation? It was certainly a possibility some labs were considering at the Clinical Genome Conference in San Francisco this year.

Hi Yemima,

we used to sequence the entire genes using Ion Torrent PGM and we are happy about the results. To be honest, we don't have an access to Illumina but it looks more accurate than Ion Torrent. But last month I believe Life Technologies has launched two technologies to reduced the indels and homopolymer errors.

Please see the following link:

https://ir.lifetechnologies.com/releasedetail.cfm?ReleaseID=798937

Best regards,

Amr