Does anyone have an experience with antibodies that detect both phosphorelated and unphosphorylated forms of proteins?

Dera all, I'm searching for antibodies that detect phosphorelated form of protein and I found some antibodies that recognize both phosphorelated and unphosphorylated forms of proteins. I just...

04 May 2020 8,639 3 View

Quantum mesh in Multi-junction solar cell at the SILVACO?

I designed a multi-junction solar cell in the Silveco Atla, which has two tunnel regions. But when I use a quantum mesh for these regions, my simulation is having trouble. Can anyone guide me?

04 May 2020 2,121 3 View

Is there any GAMS source code for solving multi-objective optimization problems (Aggregate Production Planning)?

I also have question about Energy consumption, is there any way to find each product energy consumption? Is the cost of Energy consumption increases by increasing production (number of products)?

21 September 2019 4,256 1 View

Fe-Mn-Al-Ni Shape memory alloys chemical composition?

Dear researchers, Do you think that the shape memory effect of this alloy on this chemical composition is achievable?

17 February 2019 6,953 2 View

Up to what percentage of similarity is acceptable for a graduate thesis in academic engineering programs?

The zero percentage of similarity is very ideal and most journals accept 10% to 30%, but what about the graduate engineering thesis?

28 January 2019 322 1 View

How to update boundary condition in each time step in comsol?

I have a model for electrophoresis of a particle,I need to update boundary condition in each time step. Actually I calculate boundary condition (velocity of wall) from dependent variables that...

19 January 2018 3,509 4 View

How to calculate hydrodynamic and electrostatic force acting on a particle in Comsol?

I want to model forces acting on single chared collide in nanochannel with charged walls. electrical potential diffrence set along the nanochannel and cause electroosmosis flow in channel. main...

16 December 2017 9,938 1 View

If the sample size is 322, is it right to use Smart PLS for analyzing data?

The sample size of my research is 322.Since the distribution of my data wasn't normal, I used Smart PLS to analyze data.But I heard that the sample size must be at most 200 to use Smart PLS. Is it...

28 August 2016 9,927 5 View

Mixed ANOVA or Two-way ANOVA?

If I have to count the bacterial load of 3 different stations in a lake along time (4 seasons), how should I analyse the results? Should I use Mixed ANOVA or 2-way ANOVA? Any other suggestion and why?

27 April 2016 3,662 9 View

What is the best way to reprobe another antibody on a western blot?

Currently I'm using "Restore plus western blot Stripping Buffer" for reprobing a new antibody on my blots. I am not that happy with the results because the residues of first use are still there!

02 March 2014 2,985 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View

Is there a way to extract total RNA and protein from cell culture at the same time, without denaturing protein?

Hi all, Is there a way to extract RNA and protein from the same cell culture sample? Because we need to use the protein for gelatin zymography at later time, we are looking for a method that can...

22 February 2021 5,341 5 View