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Questions related from Melika Fard
Hello, I'm planning to design a pegRNA for my prime editing experiment. I read a few relevant papers but I'm still very confused. Any help is really appreciated. Regards, Melika
12 August 2023 121 0 View
Hi guys I have a question that I would be grateful if you could help with. I did a nucleofection experiment ( LCL cells) and for one sample, I used 2 gRNAs (called 9 and 10 here). After DNA...
10 March 2022 3,047 1 View
Hello I'm growing EBV-LCL cells but they are not growing well. Any idea why this is happening? Can it be caused by the reactivation of EPV virus stressing LCL cells? Should I use EPV antigen test?...
20 July 2021 4,427 3 View
Hi guys, I'm trying to design a gRNA in https://design.synthego.com but so far have not been successful. My gene is ATM, but when I enter ATM in the gene and the genome section I don't get any...
01 January 1970 5,492 8 View
Hello everyone I'm using CRISPR technology to edit a mutated gene in human cells. I used 2 gRNAs, which resulted in the deletion of about 300bp between the two guide RNAs. This was confirmed by...
01 January 1970 302 0 View
