Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Please. Could you help be in implementing Fractal Discrete Cosine Transform algorithm in Matlab?

I do need the Matlab code of Fractal Discrete Cosine Transform (FDCT). Can anyone who has already implement this code, help me with the implementation of this transform?

24 February 2021 5,602 2 View

How to interpolate from a time-series database ?

Hi, I am having trouble with a problem, in the field of Optimal Control and the generation of optimal time-series. Let's consider a system, whose dynamics are represented by dx/dt =...

24 February 2021 9,105 2 View

What is the reason film thickness increased?

what is the reason film thickness increased when increased doping concentrations give some reasons The measured film thickness is 156, 282, 394, 548 and 681 nm for 0, 1.5, 2.5, 3.5, and 4.5 wt....

17 February 2021 8,987 5 View

Is an encrypted dataset with Microsoft SEAL compatible with Cloudsim simulator??

Hello, I would like to do homomorphic encryption using Microsoft SEAL on my dataset and then use it as an input to the Cloudsim simulator to do clustering on. Is it possible and compatible with...

16 February 2021 3,915 1 View

Queries regarding the rain flow counting algorithm?

02 February 2021 9,790 2 View

What is the type or grade of ethanol used in SOL-GEL?

I'm preparing materials that will be use in SOL-GEL to produce silica gel, I will use Tetraethyl orthosilicate (TEOS) as precursor, and ethanol as solvent , but when I search to buy ethanol, the...

01 February 2021 5,153 4 View

How to determine the genetic potential of the genotype in terms of fruit size and weight?

In addition to genetic and environmental factors, fruit size is also influenced by crop load, rootstock, orchard planting system/tree training system, pollination, water and nutrient management,...

13 January 2021 9,309 4 View

Are international conferences a dying species?

In our discipline (chemistry), annual meetings of the American Chemical Society (ACS) used to be mega-events with up to 20,000 participants. The current one will be virtual, and the organizers...

06 January 2021 1,882 5 View

Is it possible to ligate two restriction ends those have no complementary bases?

In my lab,we are trying to construct a control plasmid. So we have to remove the insert gene which has two restriction ends:one is XhoI and other one is Hind III.So after double digestion we want...

01 January 2021 3,271 4 View

How to combine two geometries in LAMMPS ?

I am trying to simulate contact angle of water on a quartz slab using LAMMPS. I have created and equilibriated the quartz slab and the water cluster separately. Now I want to create a composite...

17 February 2021 5,884 2 View

How to mesh complex geometry?

I have been trying to mesh some complex geometry and have been having issues. I have attached images of the geometry in question. This structure is very large in size but has some comparatively...

05 February 2021 6,379 5 View

Please recommend me the proper steps (blocking method) to generate Hexahedral mesh of the following geometry inside ICEM-CFD?

I am facing an issue while blocking for the geometry mentioned in the image. After blocking it ends with a triangular block due to its shape. How to create a hexahedral mesh for this geometry in...

02 February 2021 888 1 View

How to find nature of extremum of a functional? what are the mathematical prerequisites to understand it?

For a function, usually sign of second derivative (and if it is zero, even/odd index of higher order derivative whose numerical value is zero) is enough to detect whether the extreme point is...

25 January 2021 7,703 3 View

Frequency calculation with TDDFT???

Hi, I have been trying to calculate the frequency vibrations of the excited state S1 of an organic molecule (24 atoms). The geometry optimisation of the excited state converged smoothly howerver...

22 January 2021 1,463 3 View

Doubt in DFT geometry preparation?

Hi all, I am trying to perform some ab inito DFT calculations using cp2k code. I have a unit cell of 120 atoms and would like to know how to refine or reduce the number of atoms in the unit cell...

20 January 2021 6,024 3 View

In Ansys APDL the stiffened plate girder is not getting meshed after gluing! What to do?

This is a model of a stiffened plate girder. The APDL code is provided below for its geometry generation. It is not getting meshed after gluing all the volumes. If the volume is subdivided in the...

18 January 2021 4,839 4 View

How to implement truss element with no effect of compression in ansys workbench static structural?

I was trying to add ligaments in cervical spine geometry. There it is mentioned in the existing literature to add the ligaments in the geometry as truss element with no effect of compression....

03 January 2021 2,205 0 View

Hi, How can choice the most adequate mesh for a geometry on Ansys fluent?

Users of Ansys Fluent

31 December 2020 3,019 7 View

How to solve this FEM Problem in Abaqus?

Hello, I am a student and I have been trying to mesh this part but I am failing to mesh it with smaller elements because I get a warning that some of the marked elements are of poor quality...

15 December 2020 5,728 3 View