Through a Wnt inhibition assay, I have found out that my test drug, which is easily available in the market and used for Hyperlipidemia, is a good Wnt inhibitor. Now, I want to conduct an animal study. How can I determine the dose of my test drug?
Many articles are available for my drug for various diseases. Can I take doses from these articles? I need three doses: Low, Medium, and High."
Hi Ruchi. I think taking doses from the other articles would be a good place to start. You could also do a small toxicity experiment by choosing a whole range of doses and injecting 1 animal per dose per day or whatever your dosing schedule will be, to see if you find a toxic dose. This is usually done when you have no references of where to begin.
Hi Ruchi...you didn't mention what kind of model organism is going to be used in vivo studies. No. of factors have to be taken into consideration for in-vivo drug administration studies. As Kerry S Vistisen suggested, you should follow the other articles as references for test drug administration. Also, if your test drug is a repurposed drug, follow the guidelines of the FDA and compare test drugs with references to drug-dose conversion from various databases such as PubMed and Google.
The following article will help you to understand the basics of animal drug administration:
Turner PV, Brabb T, Pekow C, Vasbinder MA. Administration of substances to laboratory animals: routes of administration and factors to consider. J Am Assoc Lab Anim Sci. 2011 Sep;50(5):600-13. PMID: 22330705; PMCID: PMC3189662.
Thank you so much Brahmaji Sontyana for the answer. It will be helpful for me. I am going to use SD rats and T2DM model is HFD-STZ model.
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