Brahmaji Sontyana

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Questions related from Brahmaji Sontyana

What is the reason for not getting nitric oxide result in spleenocytes stimalated with LPS?

I stimulated the isolated splenocytes with LPS (1 ug) and incubated them for two different time points 24hrs and 48 hrs. Then checked the Nitrite levels in the culture supernatant were by using...

15 March 2023 355 0 View

How can I develop a western blot for mature IL-1 beta and caspase -1from supernatant? Can anyone share the protocol?

I am working on one of the Mtb proteins, PPE18 whether it inhibits inflammasome activation or not? For that, I am trying to develop a western blot for pro and mature forms IL-1 beta. I got a good...

18 March 2021 6,532 0 View