Is it reasonable to use stress boundaries to simulate situations with tectonic stress in FLAC3D?

Dear all I want to build a small fault model, considering the tectonic stress, the horizontal stress is relatively high.Is this the case where you can only use the stress boundary.This is a fixed...

13 July 2020 450 3 View

Should I equilibrate protein at different temperature in molecular dynamcis simulation?

I would like to determine the structural changes of my protein at different temperature (eg. 350 K). In gromacs tutorial, the protein is equilibrated using nvt and npt under normal condition (300...

09 April 2019 9,392 3 View

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05 July 2017 2,232 8 View

Is there method to extract RNA of both gram positive and gram negative bacteria?

Hi, Is there a method to extract RNA of both gram positive and gram negative bacteria in their dual-species culture? I want to perform transcriptomic analysis, so I need RNA sample with high...

01 March 2017 7,922 2 View

Is there a method to differentiate pseudomonas and salmonella on visual examination?

I want to use SEM or other microscopy method to visualize the spectral organization of these two bacteria cultured together, however, they are both gram negative and rod-shaped. Is there any other...

08 March 2015 9,029 21 View

How do I isolate rat epithelial cells?

I found some protocols on the internet and I tried them out, but they don't seem to be working. I separated the cortex from the kidney and minced and then digested the tissue with HBSS buffer...

09 April 2014 9,313 1 View

The difference between displacement and replacement

01 January 1970 1,475 2 View

What is the application of deterium excess in hydrology?

Deterium excess, or d, is defined by the following equation: d=delta(H-2)-8delta(O-18) what are the applications that have so far been reported in the literature?

01 January 1970 4,073 5 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Are there any candidates for an Einstein's bare cosmological constant?

It is commonly accepted that General Relativity has its own 'bare' cosmological constant that contributes together with the cosmological constant resulting from vacuum energy density for an...

23 February 2021 5,448 8 View

How can I transform 50 kb plasmid into ecoli?

I must put 50 kb plasmid into ecoli strain. I think 50 kb is too big for heat shock with ecoli. Is that correct? Will electroporation work? Or do I need to use cos sites and lambda phage...

22 January 2021 8,612 3 View

Does increasing the number of individuals by copy-paste of the same indvs to perform GWAS affect the results?

I am trying to perform a genome-wide association on mitogenome variants (obtained from GATK-Mutect2) vs. phenotypes by using GAPIT. The number of individuals is seven and GAPIT function does not...

14 December 2020 8,853 3 View

Weibull fading matlab codes ?

hello I tried to looking for Weibull fading channel matlab codes and i found just the following formula: h = lambda.*(-log(rand(nRx(jj),N)).^(1/beta)) my question is this codes express the...

09 December 2020 6,575 1 View

Is there any tool to extract phenotypes from facial photos?

The facial photos corresponds to various rare genetic disorders.

22 November 2020 738 1 View

Why is only kappa chain primers are used and not lambda chain primers in most of the studies to generate monoclonal antibody production?

I am trying to generate monoclonal antibodies (murine Fab, BALB/c mice) by the phage display method. Many studies focused on amplifying the kappa region of the light chain. Isn't that both kappa...

15 October 2020 9,155 4 View

Why is my molecular rotor displaying a bathochromic shift in toluene compared to MeOH or EtOH?

Hello, I am studying the photophysical properties of a molecular rotor which unexcpectedly, shows a lambda(max, absorption) = 565 nm in toluene, but a lambda (max, absorption) = 540 nm in MeOH...

15 September 2020 7,830 3 View

What statistical approach should I use for QTL-mapping of traits that are non-normally distributed and dependent?

Dear all, I have a genetic map constructed for the F2 cross and a corresponding phenotype data for parents and progeny. The problem with the phenotype data (it is the measurements of 5 biochemical...

10 September 2020 8,712 6 View

What is the best available software for HPO similarity measurement?

I would like to compare similarities of different allelic disorders based on HPO terms. As a first approach I just would like to select a few genes and look at how similar are the defined allelic...

10 September 2020 5,032 3 View