How to culture M.nematophilum?

10 February 2021 1 2K Report

I am culturing M.nematophilum with LB broth at 37oC to experiment with C.elegans but the bacteria do not seem to grow after 1 day. There has been only 1 time that the bacteria grow after 1 day. Could anyone tell me if I should change something in the condition and what is the peak time of M.nematophilum growth? Thanks

Vinanti Sarkar Castellarin

Sorry to science techie to understand ...

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