My research work is focused on study of three dimensional chromatin organization of differentially methylated regions, I need to crosslink hippocampus immediately and then lysis of cells. I am facing problem in these two steps, I would appreciate any help you could provide for me.
If you are doing your study in-vitro, you might want to pre-infuse the hippocampus with a mixture of photoactivatable crosslinkers like Psoralen and even glutaraldehyde derivatives. Then it might allow you to crosslink cells immediately in a temporal manner and possibly even locally depending on power of laser used and focusing. Once crosslinked, you can possibly preserve the three-dimensional interactions and lyse cells by grinding in liquid nitrogen with glass beads and extracting chromatin by MNase.
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