Does anyone know the ideal cell density for establishing TIB-75 xenograft in BALBc mice?

I am trying to establish TIB-75 cell line xenograft in BALBc mice.

19 January 2021 5,813 2 View

How is the power spectrum of the backscattering produced by the atmosphere on radar transmission?

If a radar send an electromagnetic wave to track some object I would expect that the returned wave (from the object) is accompanied by backscattering from atmosphere molecules. My question is, how...

17 January 2021 1,208 2 View

QPCR considerations in analyzing samples from the field?

I am attempting to plan a qPCR experiment in which I evaluate the expression of multiple genes in various brain regions of fetuses, as a function of location in utero (intrauterine position,...

31 October 2020 5,788 1 View

Can I perform immunohistochemistry on tissue after it has already been flash frozen?

I did not plan on staining but now it is a necessity. So tissue was immediately frozen using liquid nitrogen and then placed in -80 C after removal. This is human tissue and I would like to stain...

08 October 2017 4,357 3 View

What is the best way to evaluate that the AZD-7762 inhibitor is working on Chk1 upon DNA damage?

Hi! I am trying to inhibit Chk1 kinase phosporilation upon DNA damage. I was thinking on using the inhibitor AZD-7762. What is the best way to evaluate that the AZD is working? Should a western...

05 January 2016 1,464 3 View

Any advice for total protein quantification from cells suspended in FBS?

11 December 2015 9,041 3 View

Why is there no separation in PFGE?

I tried to use PFGE system CHEF II (Bio-Rad), but even on length marker (also Bio-Rad) I cannot receive any DNA separation. I used 0,5X TBE buffer, certified molecular biology agarose from...

13 May 2015 4,614 14 View

What are the best data mining tools for health care data?

We have moved into the era of "big data", and tools that have traditionally been applied to other industries are now being considered in health care. Data sources include claims data, survey data,...

24 March 2015 3,170 11 View

Are there any methods to isolate microorganism which can not grow on standard agar plates?

Hi, I'm lookong for methods for the isolation of microorganisms from liquid consortium, which could not grow on standard, solidified agar. Strains which grow on agar plates are unable to degrade...

17 November 2014 2,572 10 View

Why am I getting no PCR products in long range PCRs using cDNA as template?

08 June 2014 3,011 14 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Use of Sine voltage source in Electrical circuit with frequency domain study in COMSOL?

Dear Sir/Madam I want to use Sine voltage as a source in an RLC circuit. I want to study the driving point impedance with frequency sweep. But when I use sine voltage, I get an error like The DAE...

13 January 2021 9,826 5 View

Do Fire damages Concrete Structures Below soil?

Hi All, I am working on the rehabilitation of a fire-damaged structure. Should I consider the Column below Soil structurally deficient. visually it doesn't look like fire affected the column...

12 January 2021 5,401 3 View

What is the most efficient mouse brain weight for RNA extraction for NGS?

I have collected the brains and they are Fresh Frozen into PFC, Mid Brain and Brain Stem. I want to extract the amygdala and hypothalamus.

01 January 2021 7,833 1 View

Which form of tryptophan and tyrosine is highly soluble in water?

The solubility of tryptophan and tyrosine in water is low. Is there a very highly soluble structurally similar or derivatives of these amino acids I can use? it doesn't matter if it is...

20 December 2020 3,157 3 View

How do AFDyes(TM) compare with Alexa Fluor?

Just wondering due to the stark price difference between AFDye 594 Cadaverine (Click Chemistry Tools) and Alexa Fluor 594 Cadaverine (TFS). From what I read they are supposed to be structurally...

11 March 2020 9,532 2 View

Which is the best marker to quantify embryonic microglia in hypothalamus by flow cytometry?

I need to quantify embryonic microglia (E17) by flow cytometry. Anyone has experience in this? Thank you!

03 March 2020 8,841 3 View

What criteria is needed to consider one amino acid as hot spot?

As I checked some papers like the following one, the authors consider an amino acid as hot-spot if mutation to the alanine change the DeltaDeltaG > 2 kcal/mol. Protein-Protein...

21 October 2019 6,316 1 View

Are nations simulated by nation brands?

09 October 2019 1,587 2 View

Are nations simulated by nation brands?

06 October 2019 5,376 2 View