Does anyone know the ideal cell density for establishing TIB-75 xenograft in BALBc mice?

I am trying to establish TIB-75 cell line xenograft in BALBc mice.

19 January 2021 5,813 2 View

How is the power spectrum of the backscattering produced by the atmosphere on radar transmission?

If a radar send an electromagnetic wave to track some object I would expect that the returned wave (from the object) is accompanied by backscattering from atmosphere molecules. My question is, how...

17 January 2021 1,208 2 View

QPCR considerations in analyzing samples from the field?

I am attempting to plan a qPCR experiment in which I evaluate the expression of multiple genes in various brain regions of fetuses, as a function of location in utero (intrauterine position,...

31 October 2020 5,788 1 View

Can I perform immunohistochemistry on tissue after it has already been flash frozen?

I did not plan on staining but now it is a necessity. So tissue was immediately frozen using liquid nitrogen and then placed in -80 C after removal. This is human tissue and I would like to stain...

08 October 2017 4,357 3 View

What is the best way to evaluate that the AZD-7762 inhibitor is working on Chk1 upon DNA damage?

Hi! I am trying to inhibit Chk1 kinase phosporilation upon DNA damage. I was thinking on using the inhibitor AZD-7762. What is the best way to evaluate that the AZD is working? Should a western...

05 January 2016 1,464 3 View

Any advice for total protein quantification from cells suspended in FBS?

11 December 2015 9,041 3 View

Why is there no separation in PFGE?

I tried to use PFGE system CHEF II (Bio-Rad), but even on length marker (also Bio-Rad) I cannot receive any DNA separation. I used 0,5X TBE buffer, certified molecular biology agarose from...

13 May 2015 4,614 14 View

What are the best data mining tools for health care data?

We have moved into the era of "big data", and tools that have traditionally been applied to other industries are now being considered in health care. Data sources include claims data, survey data,...

24 March 2015 3,170 11 View

Are there any methods to isolate microorganism which can not grow on standard agar plates?

Hi, I'm lookong for methods for the isolation of microorganisms from liquid consortium, which could not grow on standard, solidified agar. Strains which grow on agar plates are unable to degrade...

17 November 2014 2,572 10 View

Why am I getting no PCR products in long range PCRs using cDNA as template?

08 June 2014 3,011 14 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

What is involved in morphological changes of SEM images of a substance?

In the sem analysis of CaCO3 nanaoparticles, the morphology images showed spherical shaped particles but the proposed morphology for these type of nanoparticles is trigonal. I prepared the...

01 March 2021 1,960 4 View

Joint behaviour in Abaqus is not captured well?

I am working on roof joint and trying to simulate the behaviour in Abaqus. I am successful in running the model but the final graph does not follow the experimental curve in the negative direction...

01 March 2021 6,661 3 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View