How to count cells of a plant tissue in mitosis manually without using a cell counter ?

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Does it exist some special technique for cutting ceramic membranes for Cross-sectional FESEM images?

Does it exist some special technique for cutting ceramic membranes for Cross-sectional FESEM or SEM images?

25 July 2024 5,482 2 View

Comment aboutire à une traduction parfaite des documents juridiques?

La traduction touche plusieurs domaines ce qui fais que le traducteur doit avoir des connaissances largement suffisante en plus de sa métrise des langues.

23 May 2024 5,751 0 View

I have an amount of PVA powder. I want to know its molecular weight. is there anyway I can understand that?

this pva that I have, works really well for electrospinning. but I don't know its molecular weight. is there any way that I can understand the molecular weight of PVA powder?

10 May 2024 3,294 5 View

what is a suitable protocol for sesame oil dry heating?

Can I sail the container during dry heating of sesame oil? I want heated it up to 170 C.

25 August 2023 6,935 4 View

Why my Au microelectrodes is not reaching a steady state current at a chronoamperogram?

I'm recording chronoamperograms at -200 mV potential in a gold 10 micrometer diameter microelectrode in TMB solution but it seems that my electrode is not reaching a steady state. The current...

09 July 2023 4,308 0 View

Likert Scaled questionnaire: in what way it can be analyzed through jamovi?

I have conducted research. Made a questionnaire. I tried to make a correlational analysis, but now there is trouble. Should I sum the total of answers and correlate it as a satisfaction with each...

11 June 2023 1,765 3 View

Mediation analysis with multiple categorical latent variables?

Is it possible to use Mplus to implement a X-M-Y mediation model, where the mediator (M) and the outcome (Y) are both categorical latent variables (defined by Latent Class Analysis)? If so,...

08 June 2023 4,350 3 View

Can anyone help me download this article?

"RTMS induces changes in FDG brain metabolism in patients with epilepsy" by Laura-Seynaeve

02 June 2023 6,113 4 View

UTAUT2 with added constructs, what statistical models can be used??

I am currently doing my undergraduate study, and I am using UTAUT2 to evaluate factors that can affect adoption of a new application we made. I have 30 sample size, all obtain using a purposive...

25 May 2023 8,594 1 View

Does ecophobia fall under the category of environmental ethics?

27 February 2023 1,821 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View