How we can dissolve theophylline derivatives for the MTT test?

I have a question about your letter, Fluorescent phosphoinositide 3-kinase inhibitors suitable for monitoring of intracellular distribution. Can you tell me how we can dissolve theophylline for...

26 January 2021 9,094 1 View

Can anybody suggest me a cheap material for surface modification of hydrophilic Titania nanoparticles?

I'm looking for a cheap material to modify Titania nanoparticles in order to use them in polymer and increase its contact angle.

13 December 2020 504 5 View

Importance of the tunnel effect on the solar cells performance?

Does the tunnel effect play a major role in the performance of solar cells? Especially when the device is heavily doped so the depletion layer can be thin enough for tunneling. Are there any...

10 December 2020 5,518 10 View

How can I find a questionnaire for Corporate identity?

My thesis is about the relation between corporate social responsibility and corporate identity. And I can not find any questionnaire about it. Can you help me?

24 July 2020 3,507 3 View

How to deal with a skewed Likert scale data with Ceiling/Flooring effects in a before-after design?

I have data of 30 subjects from a before-after design study. We used likert scale to investigate effects of changes in mood on some dependent variables. We hypothesized that the intervention would...

22 February 2020 6,496 5 View

What are the quantities per microliter of the different components needed for a reaction (1isolate of S. aureus ) to run a multiplex PCR ?

Dear colleagues, I need help. I want to run a multiplex PCR to look for enterotoxin-encoding genes in coagulase-positive StaphyloccIoccu . I have all the reagents needed to prepare the MIx PCR...

13 February 2020 7,036 2 View

Which international universities hold the Digital Transformation DBA course?

Hi dears, I want to learn more about Digital Transformation and its impact on Business model, strategy, marketing, agility and ... .I want to apply for online course at universities; is there any...

24 December 2019 3,817 11 View

I performed P-AKT western blot experiment. Even in 0 MOI, I got band. Is there any suggestion?

In any other phosphorylated proteins, in 0 MOI, I did not get band, but I got band in P-TAK.

22 July 2019 1,890 5 View

Removing a column which entirely has Zero value?

Hi! I have a very big excel sheet (6000 (row)*16000(column)), in which in some of them the entire columns have zero value. Does anybody knows how to remove those columns (just not manually)?

18 February 2019 8,960 3 View

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04 February 2019 2,154 5 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View