How can we know the Nucleic acid (DNA) denaturation in sample ?

I need to isolate DNA from an old animal skin cell. But before going for isolation of DNA. I need to clarify that the DNA is denatured or not? Please recommend me a method for that.

01 October 2020 8,265 2 View

Are all straight line shows slightly bend in nature?

Mathematics researcher

02 October 2017 9,929 3 View

Is diseaseless community always create happiness?

BIology researcher

20 August 2017 578 3 View

Are electrons (planets) and nucleus (sun, octet) surrounded in elliptical path?

Any chemistry researcher

13 August 2017 5,632 4 View

How metal-metal bond calculated in metal carbonyls? is that right or wrong?

Any inorganic chemistry student

09 August 2017 9,130 2 View

Why dont we call all the compound as cordination compounds? except hydrides and halides. because all the compound having cation and complex anion

Any researcher from inorganic chemistry

28 July 2017 8,175 2 View

Why dont we call potassium carbonate as coordination compound? its having cation and carbonate complex anion.

Any researcher from inorganic chemistry

28 July 2017 8,943 10 View

I strongly believe only two to sixth sense are present. without nothing works in the world. this is my intellectual property right

Any one from universities, colleges etc

21 July 2017 1,570 3 View

Q(pi) Countable?

We know that Q[pi] is not a field, but Q(pi) is. However, is that Q(pi) countable?

11 January 2016 8,129 23 View

Can anyone suggest the proper medium and any other specification regarding healthy NGP cell growth?

I have problem to culture NGP cells. When I trypsinized then the cells from the parent flask has gone? Please help me to find the solution.

18 February 2015 7,498 2 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

No title

Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...

08 February 2021 7,285 1 View

How define one baker unit(BU) is the amount of hydrogen peroxide catalase ?

07 February 2021 2,006 1 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View

What are some common oxidants used to oxidize iron sulfur clusters in proteins for techniques like EPR?

I have used potassium ferricyanide (which is likely the most common) extensively in the past, but I am curious to know if there are other oxidants that work well. I need to generate the oxidized...

29 January 2021 9,803 4 View

Papain Enzyme Assay...?

Hi all, I would like to know how can I estimate the activity of the enzyme, papain, which is used in reactions as crude latex extract of papaya. Please share some procedures if anybody having...

26 January 2021 569 4 View

Where can I find plants pyr-GC-MS data?

25 January 2021 5,403 2 View

Recombinant restriction enzyme: how strong should the corresponding methyltransferase expression level be?

Hello. We are planning expression of a restriction enzyme in E. coli, and wondering how much of the corresponding methyltransferase is needed to be expressed in order to protect the host genome...

23 January 2021 2,096 4 View