I work with amino- Salicylic acid derivative as a chelator for Mn . It dissolves only in DMSO and because the volume of DMSO I can put on cells is limited, the final concentration is very low comparing to the Pharmacological concentration use in mice or human (1:1000).
Now I have only 0.625 mM at Max.
For patients, It is used 6 g salt of the chelator \ 500 ml 10% glucose ,a day (slow infusion), for 4 days a week, multiple 15 weeks. How I convert it to the right concentration for my experiment taking into account:
* I put the solution on one layer of cells
* I use Free acid 99% (in DMSO) and not the salt
* I don't know the affinity of the chelator to Mn . There is no reference for Kd of the chelator. offers how to calculate that?
Bless you!
Pharmacological values have almost nothing to do with how much compound you'll need to effect cells, so I wouldn't think too much about that. Full system doses involve all ADME properties: cells you only care about protein affinity, off target affinity, aqueous solubility, and lipid solubility.
So honestly: guess. You're really just going to have to do a dosing experiment, looking at maximum dose and going down in dilutions, and measure the effect the compound has as each concentration.
Also: it might be worth just making the salt of the compound. It should be as simple as adding a stoichiometric amount of HCl or NaOH, and it'll make your studies a lot easier in the long run.