One way is to do, find the enzyme concentration using pierce BCA reagent or Bradford assay directly.
Because UNIT is based on catalytic activity of enzyme and which is in micromole /min
This can be done if you know the turnover-number of the enzyme (number of substrate molecules split or product formed per minute per enzyme molecule, this is of course equivalent to mol substrate per minute and mol enzyme). Since you know the rate of substrate conversion, you can calculate the molar concentration of the enzyme. Using the molecular mass of the enzyme, you could convert this value into a mass concentration. Note that the turnover number strongly depends on conditions, so I'd use the results of this calculation as guestimates only.
If the turnover-number is known, you can find it in BRENDA (https://www.brenda-enzymes.info/).
first you now that the unit of enzyme activity express by (unit/ml) and that mean ( one international unit (IU) of enzyme is that amount that catalyzes the formation of mirco mole of product per minute under defined conditions. ( Ref. Biochemical calculations by IRWIN H. SEGEL,)
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