Nishya Mohamed Raseek

2 Questions 4 Answers 0 Followers

Questions related from Nishya Mohamed Raseek

How can I improve the His tagged protein binding to the Co-NTA column?

I have been expressing FixAB protein(mutant) in E.coli (Nico21(DE3)). I see my protein (in SDS)is getting expressed but most of the protein is present in Flow through. I still have a little...

09 January 2018 3,736 3 View

Why there is a larger DNA band than the template plasmid size appears in 1% Agarose gel after PCR?

I'm doing a point mutation using PCR, I have seen bands in DNA gel that are larger (10000 or 8000 bp) than my template plasmid (6000 bp). Does anyone know reason for this.

18 April 2017 5,086 6 View