My experiment design requiring use of virus components from start to obtain a lentivirus that carry designated DNA to create a stable cell line, before infection we usually use a 50c.c. 100kDa centrifugal condenser to condense the virus sup. That is to condense a certain amount of sup. into 1c.c., for example, to condense 1c.c. from a starting volume of 10c.c., centrifuge for ~1500G, 5 mins, then repeat until the virus containing sup. remains with 1c.c.
The question is, can I let all the liquid go through, than just pick 1c.c. from the sup. that went through the condenser back to the upper side, to dissolve virus?
Advice been given from my lab's coworker, that envelope or the outer part of lentivirus could "dry out" during centrifugation, that's the reason why there must always have liquid in upper part of condenser. Was this also true?
This type of concentration works with some proteins like mABs but for a living virus I don't know I would always try to keep a bit of liquid to avoid drying out and also avoid over heating during centrifugation steps
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