I'm planning to clone a considerably large Drosophila  gene (~40kb) from genomic DNA into a vector with an attB site for phiC31-mediated integration into an attP landing site. I plan to use Gibson assembly of overlapping PCR fragments, which can reportedly be used to make even larger constructs (https://www.sgidna.com/ultra_kit.html). 

My question is what I would need to consider in cloning such a large insert. I've been having a hard time finding guidelines on the types of materials (vectors, cells, etc) and methods are recommended for this kind of thing.

Thanks for your help.

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