Abigail Lamb

2 Questions 6 Answers 0 Followers

Questions related from Abigail Lamb

Alkaline lysis BAC prep. Very bright low molecular weight band on gel. Residual rNTPs after RNase treatment?

I recently tried a low-cost protocol to isolate BAC DNA that I will use as substrate for multiple digests. The protocol is just alkaline lysis with RNAse in the resuspension buffer....

04 November 2016 4,213 3 View

How to clone a very large (40kb) insert?

I'm planning to clone a considerably large Drosophila gene (~40kb) from genomic DNA into a vector with an attB site for phiC31-mediated integration into an attP landing site. I plan to use Gibson...

04 May 2016 3,829 1 View