Replacement of carbon black has possible with aramid pulp for Tyre tread compound?

Is it possible to replace carbon black with some other organic fillers?

06 January 2020 1,194 1 View

Does an increase in diameter causes a greater variation in fluid flow ?

Two nozzles of different output diameters (0.20 mm and 0.25 mm) are being used to take mass measurements for an analysis over a time period for the same input pressure. It has been observed that...

20 June 2018 5,588 9 View

What can be the pretesting methods of carotid shunt balloons in production?

The carotid shunt is made up of a tube on the extremities of which the balloons are glued during the manufacturing process. Quality control (functionality test) are carried out in order to ensure...

19 June 2018 6,666 3 View

Does flow rate/discharge decrease when diameter increases?

Glue is discharged from a syringe tip at a constant pressure P through a constant time period t. The original tip is of diameter d1= 0.20 mm. A new tip is used of diameter d2 = 0.25...

29 April 2018 9,256 22 View

What are the different tests that can be done on a glued plastic tubing component?

Two plastic tubing are to be connected and glued in perpendicular for fluid flow. Can you suggest any tests that can be done to detect any leakage at the joint? See attached photo.

26 November 2017 4,643 4 View

Suggest me a simple and affordable method for measuring corrosion rate of a coated mild steel sample. Please exclude the Gravimetric method.

26 May 2017 3,254 5 View

Solar Irradiation Data (India)?

12 August 2016 4,562 5 View

How to measure the stokes shift for a material using photoluminescence spectra?

What is the importance of calculating the stokes shift in PL spectra?

11 October 2014 4,634 18 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

What pH condition required to coat APTES on silica coated Fe3O4?

It's a hydrolysis reaction, which may produce by either acid or base-catalyzed reaction. so can anybody help me with this, which condition is suitable for this reaction?

26 February 2021 1,760 8 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View