when I wanted to remove DMF from carbon dots solution. I added water equal amount and kept it inside 70-degree incubator. Precipitation was observed? I removed the precipitate by centrifugation and checked its fluorescence. it was ok. Can anybody help me out with the reason for the precipitation?
thanks,
1) it will depend on your scale and nature of the product. On a big scale, it is often possible to cleanly precipitate your product by slowly adding water to the reaction mixture, which replaces all the extractions and concentrations, you just filter your product and dry it.
2) If the extraction is unavoidable, can you use a mixture of EtOAc and heptane, say 1:1? The reason is two-fold: a) you reduce polarity of the organic phase, which helps in pushing DMF into the aqueous, and b) DMF is removed by evaporation with heptane quite easily (at this point I do not remember if it forms an azeotrope with heptane, you can check that). In general one good approach in such cases is to find a lower boiling solvent which can be azeotroped with your high boiling solvent.
I hope this helps,
DMF with Toluene easiest way to completely remove DMF from the reaction mixture. In 20 ml of DMF, i just added 30 ml of toluene evaporated at 55-60 o C
because dmf is miscible with water, it create a self assembled layers of c dot materials and excess carbonaceous material foculates and precipitate
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