Hi, I have concern about choosing the best loading control. I use three types of loading control which are 18rs, b-actin, and GAPDH. GAPDH give the most suitable findings for PPAR-a and CBEP-a genes expression. Though, most others paper use b-actin as the loading control. GAPDH considered as not stable loading control in some papers. But, there are some papers that use GAPDH as reference genes in 3T3-L1 cells. Thus, can I use GAPDH as loading control since it give the stable result, among the others?
Can we depend on our result, not the best suit loading control that were used by others?
Thank you
Asif Nakhuda
You could try their mean, even general transcriptions are quite popular HKG.
tbh there is no right answer but stability is good.
1 votes
0 thanks
- Badges
- Science method
More Samihah Zura Mohd Nani's questions See All
Similar questions and discussions