How to choose the mitochondrial targeted sequences?

10 March 2023 0 5K Report

Hi guys, I want to transfer a protein into mitochondrion, therefore, i need a mitochondrial targeted sequence(MTS). Since there are lots of MTS, and I choose the COX8A, SOD2, and OTC.

The first thing i found is the effect of these MTS are quite different: COX8A and SOD2 showed similar phenotypes, for OTC, quite different.

Then I used the COX8A and SOD2 to do further study. The second thing i found is SOD2 will induce the significant ROS generation, COX8A will not.

So now, I get confused, what kind of MTS should i take? Other things I have to consider in choosing the MTS?

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