I have sequence of Mitochondrial proteins at which ubiquitination occurs. i want to find out whether its located on MTS or not.
Hi How can this equation Ln(LA) = 1.038 + 0.89 ln(X) be applied to calculate the leaf area of a tomato? Can you explain with an example and what is the substitution of Ln and ln?
06 August 2024 2,508 2 View
Hi, I need to prepare a standard curve of H2O2 to evaluate the primary products of oxidation. H2O2 I have is 35%. How do I prepare a stock of H2O2 10 microlitere. If s.o has experience, I would be...
26 July 2024 3,460 2 View
In molecular dynamics simulation, to get FEL analysis, I got an error. My Python version is 3.10.7. My input files are made with a lower version of Python. But the final command to generate the...
23 July 2024 5,646 2 View
Hi everybody, We are trying to write a systematic review meta-analysis paper. But I could find 19 references. I think 19 references are not enough to do a meta-analysis section and it is better to...
10 July 2024 5,490 5 View
I need some articles on convective boundary please?
05 July 2024 5,131 1 View
Dear All, I want to cut PDMS sheet with thickness of 0.8 mm to rectangular shape with Graphtec cutter plotter CE6000-40. What condition do you offer? Do you offer any kind of special speed or force?
25 June 2024 8,111 1 View
I recently acquired a used Illumina HiSeq 2500 and am in need of flow cells compatible with this machine. As I am relatively new to this aspect, I would greatly appreciate any recommendations on...
22 June 2024 5,992 1 View
Dear researchers, Greetings. We have subjected pectin solution to ultrasound to produce oligosaccharides. Initially, as the ultrasound time was increased, the molecular weight decreased....
13 June 2024 881 3 View
Hello every body I use CTAB method for Plant DNA extraction and I need DNA for downstream steps. Unfortunately I have problem in my plant DNA extraction, quality and quantity not as expected, and...
10 June 2024 6,610 2 View
I would be pleased if you could share the report "Animal feed probiotic bacteria market for aquatic animals, BY REGION, 2022-2032" with me.
03 June 2024 255 5 View
I would like to do peptide map of a protein which is attached to PEG via lysines. Now the best approach would be to de-PEGylate it but then do the peptide map. But there is little literature on...
14 July 2024 5,634 2 View
i have been trying to differentiate primary human myoblasts into myotubes. i have early pssage (p2) and old pssage (p14). Hoth with same outocmes. the myoblasts have been growing in 20% fcs,...
27 February 2024 5,393 1 View
We developed a rapid viability test for plant seeds in which yeasts metabolize organic compounds that leach from seeds (dependent on the physiological age of the seed) and thereby reduce our...
01 February 2024 2,879 2 View
I am using Proteome Discoverer 2.3 to analyze TMT-labeled and phospho-enriched samples, but I am not getting any protein matches. I know there are relatively little peptides in these samples since...
17 January 2024 6,270 0 View
Hello, I am looking for suggestions for labeling proteins lacking any cysteins and lysines. While, SDS mutation to add a cysteine or lysine are possible options. I am looking for suggestions...
29 November 2023 7,740 1 View
When I calculate the IC50 of natural products on cancer cell and normal cells between concentration of 0.3125 and 40ug/ml using MTS, the inhibition curve is sigmoidal on cancer cells not on normal...
21 November 2023 8,656 2 View
The adoption of DNA markers, which allows for thorough gene identification of progeny at each generation, accelerates the pyramiding process. However, in developing countries, we employ...
24 October 2023 9,789 0 View
Dear All, I want to make a side-chain stapling of lysine (or its non-standard amino acid derivatives). How to make a topology file for this kind of side-chain stapling (I believe, it is not...
19 October 2023 8,280 2 View
Hi all, I wonder the reason for deleting C-term lysine during antibody engineering? Thanks
18 September 2023 3,199 5 View
I am using a magnetic separation based isolation kit for mouse neutrophils and the protocol mentions 2 incubation on ice for 15 min each. I am aware that low temperature pre-activate the...
05 September 2023 6,293 0 View