How to check the proper folding of in vitro transcribed RNA?

29 August 2021 1 978 Report

I have transcribed one type of structural RNA (~500 nt long). For the downstream experiment, I need to confirm its proper folding. In literature, I have found people just heat the RNA at 95 C and chill it in ice for refolding. Do you have any idea, how to check the difference in the folding of these two types of RNAs (just in vitro transcribed and heat treatment after transcription)?

Md Solayman

Hi Vipin Bhardwaj,

Thank you so much for your reply. One of my labmates suggested using DLS. If I don't find the answer then I may need to use an alternative method.

Cheers

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